Protein_Domain

Part:BBa_K105002

Designed by: Sandra Dittmann   Group: iGEM08_ESBS-Strasbourg   (2008-10-12)

TUP1 - repressor domain for transcription factors in yeast

This BioBrick is the N-terminal repression domain of the yeast general repressor TUP1.<br\n> In artificial systems TUP1 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective repression of genes with a promoter containing this bait sequence can be achieved.

<br\n> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
    Illegal NheI site found at 469
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 257


[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/eukaryote/yeast
Parameters
None